State of Iron in the Archaebacterium Methanosarcina barkeri Grown on Different Carbon Sources as Studied by Mössbauer Spectroscopy
نویسنده
چکیده
Archaebacterium, Methanosarcina, Methanol, Iron, Mössbauer, Ferredoxin Cells of the archaebacterium Methanosarcina barkeri were grown strictly anaerobic in defined media. 57Fe-Mössbauer Spectroscopy of methanol grown cells oxidized in the presence of air demonstrated only the state of Fe3+ whereas active cells under reducing conditions offered spectra of two additional iron sites in the Fe2+ state. Furthermore the 57Fe hyperfine interaction data at different temperatures gave evidence that cells cultivated with methanol or acetate as substrate contained ferredoxin like compounds.
منابع مشابه
Diazotrophy and Nitrogenase Activity in the Archaebacterium Methanosarcina barkeri 227.
Nitrogen fixation (diazotrophy) has recently been demonstrated in several methanogenic archaebacteria. To compare the process in an archaebacterium with that in eubacteria, we examined the properties of diazotrophic growth and nitrogenase activity in Methanosarcina barkeri 227. Growth yields with methanol or acetate as a growth substrate were significantly lower in N(2)-grown cultures than in N...
متن کاملConservation of structure in the human gene encoding argininosuccinate synthetase and the argG genes of the archaebacteria Methanosarcina barkeri MS and Methanococcus vannielii.
The DNA sequences of the argG genes of Methanosarcina barkeri MS and Methanococcus vannielii were determined. The polypeptide products of these methanogen genes have amino acid sequences which are 50% identical to each other and 38% identical to the amino acid sequence encoded by the exons of the human argininosuccinate synthetase gene. Introns in the human chromosomal gene separate regions whi...
متن کاملGenetic Code: Introducing Pyrrolysine
Monomethylamine methyltransferase of the archaebacterium Methanosarcina barkeri contains a novel amino acid, pyrrolysine, encoded by the termination codon UAG. Initial studies suggest that pyrrolysine may be co-translationally inserted during protein synthesis, probably by a mechanism analogous to that operating during selenocysteine incorporation.
متن کاملCo-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture
Methanosaeta harundinacea and Methanosarcina barkeri, known as classic acetoclastic methanogens, are capable of directly accepting electrons from Geobacter metallireducens for the reduction of carbon dioxide to methane, having been revealed as direct interspecies electron transfer (DIET) in the laboratory co-cultures. However, whether their co-occurrences are ubiquitous in the iron (III)-reduci...
متن کاملEffect of Nickel Levels on Hydrogen Partial Pressure and Methane Production in Methanogens
Hydrogen (H2) consumption and methane (CH4) production in pure cultures of three different methanogens were investigated during cultivation with 0, 0.2 and 4.21 μM added nickel (Ni). The results showed that the level of dissolved Ni in the anaerobic growth medium did not notably affect CH4 production in the cytochrome-free methanogenic species Methanobacterium bryantii and Methanoculleus bourge...
متن کامل